Anti-hyperglycemic activity of the herbo-mineral siddha preparation in alloxan induced diabetic rats

To evaluate the anti hyperglycemic effect of this new Herbo -Mineral preparation in alloxan induced albino diabetic rats and Acute toxicity studies for this new Herbo -Mineral preparation. Experimental Rats were allowed to fast 24 hrs prior to diabetes ,induced by administration of a single dose of( 120 mg/kg body weight) Alloxan mono hydrate intraperitoneally ,after 24 hours blood was collected from the tail vein and blood glucose was determined using electronic Glucometer. This was done in three groups, Gr-1 were fed only Carboxy Methyl Celluose (CMC) sodium orally,0.5 ml for 15 days Gr-II were given alloxan Intra Peritonally and Gr-III: After overnight fasting, the new product (10 mg/daily) orally,0.5 ml in Carboxy Methyl Celluose (CMC) sodium for 15 days. The animals were tested after 48 hours and other biochemical parameters were done to assess for toxicity profile by using standard kit methods .Chi-square,mean and SD was calculated using SPSS software. Alloxan has been shown to produce hyperglycemia .Restoration of normal levels of blood glucose by the product was 75% in group III which categorically an excellent figure in small animals. This reversion of the blood Glucose levels to normalcy due to reversion of the damaged pancreatic beta cells activity and the alloxan induced diabetes may be due to complete destruction of beta cells in the animals. No mortality with in a period of 48 hours and the bio chemical parameters were totally normal. The new Herbo -mineral combinations have effects to regulate blood glucose level and other parameters.